id,title,description,date_created,date_modified,date_published,original_publication_date,publication_doi,provider,is_published,reviews_state,version,is_latest_version,preprint_doi,license,tags_list,tags_data,contributors_list,contributors_data,first_author,subjects_list,subjects_data,download_url,has_coi,conflict_of_interest_statement,has_data_links,has_prereg_links,prereg_links,prereg_link_info,last_updated
72zcs_v1,Preclinical Investigation of Ultrasound-Guided Focused Ultrasound Therapy for Sacroiliac Joint Low Back Pain,Ultrasound guided HIFU lesions were performed on the lumbar spine and sacrum of three swine to evaluate evidence of neural ablation and safety.  Lesions were confirmed with pathology post necropsy.,2025-03-07T18:52:08.938979,2025-03-17T16:51:23.958211,2025-03-17T16:48:35.580129,2025-01-19T05:00:00,https://doi.org/10.1136/rapm-2024-105809,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/72zcs_v1,CC-By Attribution 4.0 International,,[],Amitabh Gulati,"[{""id"": ""93zqu"", ""name"": ""Amitabh Gulati"", ""index"": 0, ""orcid"": ""0000-0003-1654-8639"", ""bibliographic"": true}]",Amitabh Gulati,Medicine and Health Sciences; Medical Specialties; Anesthesiology,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}, {""id"": ""59bacc5854be81033c4e4ad6"", ""text"": ""Medical Specialties""}, {""id"": ""59bacc5c54be81033c4e4b88"", ""text"": ""Anesthesiology""}]",https://osf.io/download/67cb408b5288a613bdfd8a8f,1,"consultant for Medtronic, AIS Healthcare, SPR Therapeutics, Nalu Medical, Hinge Health, Menda Health, Neurovasis",not_applicable,not_applicable,[],,2025-04-09T21:06:20.392332
jy2e7_v1,Ultrasound Triggered Gelation to  Treat Discogenic Lower Back Pain,"Motivation: Lower back pain (LBP) is one of the leading causes of global disability. LBP and related radicular leg pain are closely linked to intervertebral disc (IVD) degeneration, which accounts for approximately 40% of the estimated 619 million LBP cases worldwide. Currently, there are two diametrically opposed treatment options for this condition: conservative physiotherapy to provide temporary relief, or major surgical intervention. Neither has proven to provide suitable long-term outcomes. Emerging strategies focus on injectable biomaterials to provide structural support and facilitate tissue repair, although they are still largely experimental and face several limitations, including limited integration with native tissue. Moreover, the implant formation mechanism may be depth-limited (light curing) or time-constrained (self-curing). 

Aim: The objective of this research is to demonstrate a new option to restore spinal function through the use of extracorporeal ultrasound to remotely trigger in situ implant formation on demand, such that the clinician can control the process with the timing and location of their choosing. The system concept centers around an implant precursor material consisting of an anionic polysaccharide matrix seeded with thermally sensitive liposomes. Ultrasound-mediated heating on the order of 3-5 degrees above normal body temperature triggers the release of crosslinking species from the liposomes, thereby initiating hydrogel formation.    
 
Methods: Candidate polysaccharide and liposome formulations were evaluated for their injectability, loading efficiency, and post-gelation mechanical properties. Ultrasound parameters (frequency, pressure, duty cycle) were optimized for targeted heating efficiency. Techniques for treatment process monitoring and control were independently investigated using thermometry and acoustic cavitation emissions. The material constructs and ultrasound protocols were used together in a series of proof-of-concept experiments using ex vivo bovine IVD specimens, with biomechanical analysis across three states: intact, degenerated, and after ultrasound-triggered implant formation.

Results: Extensive testing revealed an optimized implant precursor material consisting of sodium alginate (1.5 wt/v%) seeded with calcium-loaded liposomes (157±9 nm) to enable hyperthermia-triggered release and glass microspheres (6 wt/v%) to ensure preferential ultrasound absorption for safe heating. No significant difference was found between hydrogels heated with an incubator or ultrasound, suggesting comparable calcium release between both methods. Optimal ultrasound parameters for precursor gel heating were found to be 0.95 MHz, 1.6 MPa (peak negative), and 87% duty cycle. Automated treatment control using temperature or cavitation emission measurements both were successfully implemented, with cavitation being preferable for non-invasive implementation. Proof of concept experiments indicated partial restoration of biomechanical function in ex vivo bovine IVDs, with implant material well-integrated into the disc tissue, and without material herniation.

Conclusion: We have demonstrated the feasibility of ultrasound-guided hydrogel gelation in situ. These results offer promise for treating spinal disc degeneration, with continued refinement of materials and protocols essential for achieving robust in-disc efficacy.",2024-12-14T10:09:56.635128,2024-12-14T12:02:43.042689,2024-12-14T12:02:22.683465,,,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/jy2e7,CC-By Attribution 4.0 International,,[],Veerle Brans; Anna Constantinou; Luca Bau; Constantin Coussios; Molly Stevens; Michael Gray; Matthew Kibble; Nicolas Newell,"[{""id"": ""bm2a4"", ""name"": ""Veerle Brans"", ""index"": 0, ""orcid"": null, ""bibliographic"": true}, {""id"": ""x6g3m"", ""name"": ""Anna Constantinou"", ""index"": 1, ""orcid"": null, ""bibliographic"": true}, {""id"": ""v3amz"", ""name"": ""Luca Bau"", ""index"": 2, ""orcid"": null, ""bibliographic"": true}, {""id"": ""grf4k"", ""name"": ""Constantin Coussios"", ""index"": 3, ""orcid"": null, ""bibliographic"": true}, {""id"": ""5wmzx"", ""name"": ""Molly Stevens"", ""index"": 4, ""orcid"": null, ""bibliographic"": true}, {""id"": ""f7mgc"", ""name"": ""Michael Gray"", ""index"": 5, ""orcid"": ""0000-0002-3245-3296"", ""bibliographic"": true}, {""id"": ""wgczh"", ""name"": ""Matthew Kibble"", ""index"": 6, ""orcid"": null, ""bibliographic"": true}, {""id"": ""sc4qe"", ""name"": ""Nicolas Newell"", ""index"": 7, ""orcid"": null, ""bibliographic"": true}]",Veerle Brans,Engineering; Materials Science and Engineering; Biomedical Engineering and Bioengineering,"[{""id"": ""59bacc5654be81033c4e4a6f"", ""text"": ""Engineering""}, {""id"": ""59bacc5854be81033c4e4ad7"", ""text"": ""Materials Science and Engineering""}, {""id"": ""59bacc5a54be81033c4e4b3c"", ""text"": ""Biomedical Engineering and Bioengineering""}]",https://osf.io/download/675d59966cb843fc3ef7c15c,0,,no,not_applicable,[],,2025-04-09T21:06:15.356151
uy9be_v1,Focused ultrasound and immunotherapy for melanoma brain metastases,"Using a “two-site” model of melanoma, determine the experimental therapeutic efficacy (i.e. tumor growth control, improved survival, and CD8 T cell recruitment) of αCD40 administration against extracranial and intracranial tumors. Determine whether enhancing delivery of αCD40 to the intracranial tumor via BTB opening with FUS and MBs enhances experimental therapeutic efficacy (i.e. tumor growth control, improved survival, and CD8 T cell recruitment). Determine whether thermal ablation of the extracranial tumor in the presence of αCD40 enhances experimental therapeutic efficacy for one or both tumors (i.e. tumor growth control, improved survival, and CD8 T cell recruitment). Determine whether, in the context of thermal ablation of the extracranial tumor + αCD40, BTB opening with FUS and MBs enhances experimental therapeutic efficacy in the intracranial tumor (i.e. tumor growth control, improved survival, and CD8 T cell recruitment).",2024-10-30T18:31:24.024299,2024-10-30T18:36:26.592285,2024-10-30T18:32:53.318095,,,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/uy9be,CC-By Attribution 4.0 International,,[],Richard J Price; Vinton WT Cheng,"[{""id"": ""xsk3r"", ""name"": ""Kelsie Timbie"", ""index"": 0, ""orcid"": null, ""bibliographic"": false}, {""id"": ""gesu4"", ""name"": ""Richard J Price"", ""index"": 1, ""orcid"": null, ""bibliographic"": true}, {""id"": ""xykt2"", ""name"": ""Vinton WT Cheng"", ""index"": 2, ""orcid"": null, ""bibliographic"": true}]",Kelsie Timbie,Medicine and Health Sciences,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}]",https://osf.io/download/67227b85a9f0e913a51de374,0,,no,not_applicable,[],,2025-04-09T21:06:19.363964
7pn9r_v1,Durable modulation of deep brain circuits for treatments of anxiety-related disorders,"Mental disorders affect nearly every fifth person. In about one third of the cases, there is no adequate treatment. Circuit modulation with low-intensity ultrasound provides new noninvasive and targeted treatment option. Low-intensity, sustained ultrasound promotes circuit reorganization specifically at the desired deep brain targets, thus providing the necessary reset. In this work, we will quantify the magnitude and duration of these neuroplastic effects, and apply the stimuli to the amygdala to reduce anxiety in primates. The approach, based on 650 kHz ultrasound, is projected to translate into clinical trials performed on the Insightec brain system.",2024-10-28T16:29:40.418238,2024-10-28T16:38:01.257573,2024-10-28T16:37:46.116201,,,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/7pn9r,CC-By Attribution 4.0 International,,[],Jan Kubanek,"[{""id"": ""d4nzh"", ""name"": ""Jan Kubanek"", ""index"": 0, ""orcid"": ""0000-0003-0527-5559"", ""bibliographic"": true}]",Jan Kubanek,Medicine and Health Sciences; Life Sciences; Engineering,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}, {""id"": ""59bacc5654be81033c4e4a6e"", ""text"": ""Life Sciences""}, {""id"": ""59bacc5654be81033c4e4a6f"", ""text"": ""Engineering""}]",https://osf.io/download/671fbc003e0ee50920bfa310,0,,,,null,,2025-04-09T21:06:18.447857
8dj5y_v1,Towards a Spatial Understanding: Transcriptome Profiling of the Tumor Landscape After Ablative Focused  Ultrasound Regimens,"Ablative Focused ultrasound (FUS) therapies are promising non-invasive techniques for localized tumor ablation that may additionally enhance systemic antitumor immunity. However, the spatial distribution of immune responses following these treatments remains limited. This study employs digital spatial transcriptome profiling to compare transcriptional changes in periablative versus distal, untargeted, regions of tumors after FUS treatement. We identified significant gene expression changes in tumors treated with either mechanical or thermal destruction modalities compared to controls, with an emphasis on upregulation of RNA processing and ribosome biogenesis pathways. Surprisingly, minimal transcriptional differences were observed between periablative and distal regions, suggesting that FUS-induced transcriptional regulation isn’t limited to the lesion periphery. Cellular deconvolution analyses revealed increased proportions of Th17 cells, dendritic cells, and CD8+ T cells in FUS-treated tumors, pointing to enhanced immune activation. Our results suggest that both mechanical and thermal FUS elicit widespread tumor transcriptional modulation and highlight the need for further investigation into their systemic and spatial bioeffects.",2024-10-01T19:17:48.476380,2024-10-02T14:00:21.564461,2024-10-02T13:59:54.185924,,,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/8dj5y,CC-By Attribution 4.0 International,,[],Awndre Gamache; Frederic PADILLA; Timothy N.J. Bullock,"[{""id"": ""fwm6v"", ""name"": ""Awndre Gamache"", ""index"": 0, ""orcid"": null, ""bibliographic"": true}, {""id"": ""84igd"", ""name"": ""Frederic PADILLA"", ""index"": 1, ""orcid"": ""0000-0003-2452-9497"", ""bibliographic"": true}, {""id"": ""wxau2"", ""name"": ""Timothy N.J. Bullock"", ""index"": 2, ""orcid"": null, ""bibliographic"": true}]",Awndre Gamache,Medicine and Health Sciences; Medical Sciences,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}, {""id"": ""59bacc5654be81033c4e4a7b"", ""text"": ""Medical Sciences""}]",https://osf.io/download/66fc4aeb52799181d75cc782,0,,no,not_applicable,[],,2025-04-09T21:06:15.830494
ufgh8_v1,Fabrication and Testing of a PET insert for Simultaneous PET/FUS/MRI,"Glioblastoma (GB) is the most common brain cancer and has limited survivability with a mean survival time of 15 months and an overall survival of less than 7 % after 5 years. Impediments to delivery of large molecule (e.g. antibodies) and cell therapeutics to a tumor include the blood–brain barrier (BBB) and the blood‐tumor barrier (BTB) which prevent large solutes from crossing from circulating blood into the extracellular fluid of the central nervous system. Recently, methodology has been developed to use focused ultrasound (FUS) in conjunction with microbubbles to temporarily disrupt the BBB/BTB. FUS units have been developed that can be used in the high magnetic fields that are part of a MRI scanners. The MRI is used to locate the brain tumor to determine where FUS energy will be applied to open the BBB/BTB. However, it is difficult using current technologies to determine the optimum relative timing and dosages of drugs/cells and application of FUS. We have previously shown using PET imaging of a [89Zr]‐mCD47 to GB in a mouse model, that antibody binding by a tumor is significantly enhanced if the mCD47 antibody is injected 15 min after BBB/BTB disruption, rather than injection before BBB/BTB disruption.1 However, this study was done using two fixed time points. The animals had to be transported between different scanners which prevented us from a continuous measurement of tumor uptake and washout of antibody. We propose to build a unique trimodal MRI/FUS/PET system that would permit real‐time measurement of influx of radiolabeled therapeutic antibodies or cells and allow us to optimize the timing of FUS relative to injection of therapeutics. It will allow whole‐body mouse imaging so we will be able to measure in vivo biodistribution as well. To the best of our knowledge, no such system exists anywhere. However, our group has previously published simulation studies that show that it is feasible to use a PET insert in conjunction with MRI and FUS in the setting of tumor ablation in the brain",2024-06-14T18:14:49.574628,2024-06-14T18:18:45.776898,2024-06-14T18:18:23.036539,,,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/ufgh8,CC-By Attribution 4.0 International,PET-MRI-FUS,"[""PET-MRI-FUS""]",Stuart S Berr; Antonio Gonzalez,"[{""id"": ""xsk3r"", ""name"": ""Kelsie Timbie"", ""index"": 0, ""orcid"": null, ""bibliographic"": false}, {""id"": ""nczex"", ""name"": ""Stuart S Berr"", ""index"": 1, ""orcid"": null, ""bibliographic"": true}, {""id"": ""nb7z5"", ""name"": ""Antonio Gonzalez"", ""index"": 2, ""orcid"": null, ""bibliographic"": true}]",Kelsie Timbie,Medicine and Health Sciences; Engineering; Biomedical Engineering and Bioengineering,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}, {""id"": ""59bacc5654be81033c4e4a6f"", ""text"": ""Engineering""}, {""id"": ""59bacc5a54be81033c4e4b3c"", ""text"": ""Biomedical Engineering and Bioengineering""}]",https://osf.io/download/666c88a565e1de5b09893b61,0,,no,no,[],,2025-04-09T20:49:59.592622
vy247_v1,Development of a contrast-enhanced ultrasound guided high intensity focused ultrasound system for coagulation of liver parenchyma,"BACKGROUND:The liver is the most common organ injured in blunt abdominal trauma and makes up roughly 5% of all trauma admissions. Current treatments are invasive and resource-intensive, which may delay care. We aim to develop and validate a contrast-enhanced ultrasound (CEUS)guided noninvasive tool to treat liver lacerations at the bedside.
METHODS: Two 1.8 MHz high-intensity focused ultrasound (HIFU) elements were coupled to a C1-6 diagnostic ultrasound probe and a Logiq E10 scanner (GE HealthCare) utilizing a custom enclosure for co-registered imaging and ablation. A phantom was created from polyacrylamide gel combined with thermochromic ink whose color changes above biological ablative temperatures (60 °C). The HIFU wave was focused approximately 0.5 cm below the surface utilizing a 50% duty cycle generating 11.9 MPa for 20, 30, 40, 50, and 60s. Experiments were repeated on ex vivo chicken livers in a water bath. Finally, the livers of 4 live swine underwent up to 6 CEUS-guided treatments using parameters optimized from in vitro work.
RESULTS : Treatment of the phantom between 20-60s, produced ablation sizes from 0.016 to 0.4 cm3. The relationship between time and size was exponential (R2 = 0.992). Ablation areas were also well visualized on with ultrasound imaging. The ex vivo liver ablation size at 20s was 0.37 cm3, at 30s was 0.66 cm3, and at 100 s was 5.0 cm3. For the in-vivo swine experiments, the average ablation area measured 2.0x0.75 cm with a maximum of 3.5x1.5 cm. CEUS was utilized with the contrast agent Definity (Lantheus) for identification of lacerations as well as immediate post operative evaluation of therapy.
CONCLUSION: These experiments demonstrate the feasibility of CEUS guided transdermal HIFU ablation and the time-dependent size of ablation. This work warrants future investigations into using ultrasound to detect active bleeding and HIFU to coagulate grade III and IV liver laceration.",2024-06-11T13:59:20.237025,2024-06-14T18:19:23.096471,2024-06-14T18:19:08.107556,,,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/vy247,CC-By Attribution 4.0 International,,[],Alexander Tam; Kaizer Contreras; Fari Fall; Adam Maxwell; Ji-Bin Liu; Flemming Forsberg; Eli Vlaisavljevich; Allison Goldberg; Tania Siu Xiao; Cristina Kuon Yeng Escalante,"[{""id"": ""heqpy"", ""name"": ""Alexander Tam"", ""index"": 0, ""orcid"": null, ""bibliographic"": true}, {""id"": ""942cq"", ""name"": ""Kaizer Contreras"", ""index"": 1, ""orcid"": null, ""bibliographic"": true}, {""id"": ""7md9n"", ""name"": ""Fari Fall"", ""index"": 2, ""orcid"": null, ""bibliographic"": true}, {""id"": ""94g27"", ""name"": ""Adam Maxwell"", ""index"": 3, ""orcid"": null, ""bibliographic"": true}, {""id"": ""5ckrh"", ""name"": ""Ji-Bin Liu"", ""index"": 4, ""orcid"": null, ""bibliographic"": true}, {""id"": ""2bsjh"", ""name"": ""Flemming Forsberg"", ""index"": 5, ""orcid"": null, ""bibliographic"": true}, {""id"": ""ykp5j"", ""name"": ""Eli Vlaisavljevich"", ""index"": 6, ""orcid"": null, ""bibliographic"": true}, {""id"": ""usfc2"", ""name"": ""Allison Goldberg"", ""index"": 7, ""orcid"": null, ""bibliographic"": true}, {""id"": ""ghcuy"", ""name"": ""Tania Siu Xiao"", ""index"": 8, ""orcid"": null, ""bibliographic"": true}, {""id"": ""b463e"", ""name"": ""Cristina Kuon Yeng Escalante"", ""index"": 9, ""orcid"": null, ""bibliographic"": true}]",Alexander Tam,Engineering; Biomedical Engineering and Bioengineering; Biomedical Devices and Instrumentation,"[{""id"": ""59bacc5654be81033c4e4a6f"", ""text"": ""Engineering""}, {""id"": ""59bacc5a54be81033c4e4b3c"", ""text"": ""Biomedical Engineering and Bioengineering""}, {""id"": ""59bacc5b54be81033c4e4b75"", ""text"": ""Biomedical Devices and Instrumentation""}]",https://osf.io/download/6668583cd835c431ff4ce4c3,1,"Grant support, equipment support, contrast agent support and speaker fees from GE Healthcare. Grant support,  contrast agent support and speaker fees for Lantheus Medical Imaging. Equipment support from Siemens. Contrast agent support from Bracco. Various authors provide consulting services to Bracco, Exact Therapeutics, Longeviti Neuro Solutions, GE Healthcare, SonoThera.",no,no,[],,2025-04-09T20:50:06.754601
wm7yt_v1,Investigation of low-intensity focused ultrasound for human pain management,"The insula is an intriguing target for pain modulation. Unfortunately, it lies deep to the cortex making spatially specific noninvasive access difficult. Here, we leverage the high spatial resolution and deep penetration depth of low-intensity focused ultrasound (LIFU) to non-surgically modulate the anterior insula (AI) or posterior insula (PI) in humans for effect on subjective pain ratings, electroencephalographic (EEG) contact heat–evoked potentials, as well as autonomic measures including heart-rate variability (HRV). We also test and offline protocol to determined the longevity of effects on the CHEP and pain intensity",2024-05-20T16:26:45.845811,2024-05-22T17:52:01.385403,2024-05-22T17:51:28.981271,2024-05-01T04:00:00,https://doi.org/10.1097/j.pain.0000000000003171,focusarchive,1,accepted,1,1,https://doi.org/10.31225/osf.io/wm7yt,CC-By Attribution 4.0 International,Low intensity focused ultrasound; human; neuromodulation; pain,"[""Low intensity focused ultrasound"", ""human"", ""neuromodulation"", ""pain""]",wynn legon,"[{""id"": ""czs92"", ""name"": ""wynn legon"", ""index"": 0, ""orcid"": ""0000-0002-8761-3496"", ""bibliographic"": true}]",wynn legon,Medicine and Health Sciences; Translational Medical Research,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}, {""id"": ""59bacc5654be81033c4e4a79"", ""text"": ""Translational Medical Research""}]",https://osf.io/download/664b79c92f167d124a0e7b93,0,,no,no,[],,2025-04-09T20:49:46.414328
35s4p_v1,Enhancing delivery of molecular therapies to the CNS for genetic neurological disorders,Summary of results from study investigating the use of focused ultrasound blood-brain barrier opening to improve delivery of a lipid nanoparticle based mRNA therapy.,2024-04-30T19:39:41.512911,2024-07-12T17:28:53.322235,2024-06-14T18:19:36.793215,,,focusarchive,0,withdrawn,1,1,https://doi.org/10.31225/osf.io/35s4p,CC-By Attribution 4.0 International,,[],Nick Todd,"[{""id"": ""3bx24"", ""name"": ""Nick Todd"", ""index"": 0, ""orcid"": ""0000-0003-2588-6330"", ""bibliographic"": true}]",Nick Todd,Medicine and Health Sciences; Translational Medical Research,"[{""id"": ""59bacc5654be81033c4e4a6d"", ""text"": ""Medicine and Health Sciences""}, {""id"": ""59bacc5654be81033c4e4a79"", ""text"": ""Translational Medical Research""}]",,0,,no,no,[],,2025-04-09T20:50:01.497986
ybd96_v1,Final progress report,"This is a progress report submitted to the FUS Foundation on the project entitled ""Focused ultrasound mediated gene therapy for treating spinal muscular atrophy with spatiotemporal precision""",2024-04-14T00:18:32.229994,2024-07-12T17:29:13.962394,2024-06-14T18:19:29.705451,,,focusarchive,0,withdrawn,1,1,https://doi.org/10.31225/osf.io/ybd96,CC-By Attribution 4.0 International,,[],Guosong Hong,"[{""id"": ""4brsq"", ""name"": ""Guosong Hong"", ""index"": 0, ""orcid"": ""0000-0002-8858-4471"", ""bibliographic"": true}]",Guosong Hong,Engineering; Materials Science and Engineering,"[{""id"": ""59bacc5654be81033c4e4a6f"", ""text"": ""Engineering""}, {""id"": ""59bacc5854be81033c4e4ad7"", ""text"": ""Materials Science and Engineering""}]",,0,,no,not_applicable,[],,2025-04-09T20:49:59.011523